Chiba iGEM 2007 Marimo.

Presentation on theme: "Chiba iGEM 2007 Marimo."— Presentation transcript:

1 Chiba iGEM 2007 Marimo

2 What the hell is Marimo? Marimo (Japanese: 毬藻 marimo), also known as Cladophora ball, Lake ball, or Moss Balls in English, is a species of filamentous green algae (Chlorophyta) found in a number of lakes in the northern hemisphere. blabla…

3 To make a bacterial Marimo
１ ２ ３ まりも完成

4 Our System Gather bacteria Stick bacteria together
Quorum Sensing Stick bacteria together flagella: fliC gene Control size (advanced) Tune Quorum Sensing

5 Detailed Design Ｓ Ｒ ＡＨＬ

6 Detailed Design: Sender Cell
Constitutively express flagella Produce AHL

7 Detailed Design: Reciever Cell
LuxP > flagella no AHL > aiiA ??

8 Detailed Design: Overview
①Ｓｅｎｄｅｒは常に鞭毛が生えていて、ＡＨＬを生産する。 ②ＲｅｃｅｉｖｅｒはＡＨＬ存在下で鞭毛が生えて、ＡｉｉＡによりＡＨＬを分解する。 ③鞭毛にはヒスタグが付いているのでニッケルイオンを仲介としてくっつく。 Ｓ Ｒ ＡＨＬ

9 Quorum sensing, FliC-his gene
Experiments & Results Quorum sensing, FliC-his gene

10 Experiments Quorum Sensing Flagella (fliC) engeneering
modify? for a lab-class 自由溶液 size-tunable Flagella (fliC) engeneering

11 Quorum Sensing in a liquid…
To make the AHL conc. gradation Sender(=AHL producer) Problem Senderを固める（拡散させない） 必要量のAHLを出すSenderの数 AHL拡散 Problem solvent’s viscosity AHL溜る -> 分解する(aiiA)

12 Size Control Super Sender Cell Super Reciever Cell Produce more AHL
Sensitive

13 To Make a Marimo Check the flagella adsorption w/Quorum sensing
2-dimensional plate 3-dimensional (marimo) Size control test

14 Controling Marimo Size

15 What we learned

16 Acknowledgements