YMC Pro Series of ODS Columns Hydrosphere C18 Pro C18 Pro C18 RS Silica ultra pure ultra pure ultra pure Particle size 2,3,5mm 2, 3, 5mm 3,5mm Pore size 120A 120A 80A Carbon content 12% 16% 22% Bonded phase monomeric C18 monomeric C18 polymeric C18 Endcapping completely endcapped completely endcapped completely endcapped Features hydrophilic surface excellent reproducibility high durability

Analysis of Basic Compounds (2) - Comparison with Competitors - Pyridine Amitriptyline Diphenhydramine Pro Series ○ Brand B2 △ Brand D4 × Brand F1 Brand I5 Brand J1 ○：Tf≦1.5 △：1.5＜Tf≦2.0 ×：Tf＞2.0 Pro C18 RS Brand J1 Tf(4) 1.16 Tf(4) 6.18 1. Desipramine 2. Diphenyl (I.S.) 3. Imipramine 4. Amitriptyline

Eluent : methanol/water (80/20) Comparison of Separative selectivity (2) ー Hydrophobic Selectivity Graph chartー ProシリーズODSカラムと他社カラムの比較を示す。 他社カラムには、親水性化合物分離用カラムから耐酸・耐アルカリ性の高いODSカラムまで 含まれます。 k’（Amylbenzene） Eluent : methanol/water (80/20)

Comparison of Separative Selectivity (3) ー Plane Recognitive Ability Graph chart ー ODSのリガンド密度が高いカラムほど平面認識能は高くなります。 しかし、リガンド密度がさほど高くないカラムでも平面認識能が大きくなる 場合があります。これは、充填剤表面のエンドキャッピングが不十分 な場合、平面認識能が大きくなる傾向にあるためだと考えられます。 α（o-Terphenyl / triphenylene） Eluent : methanol/water (80/20)

Eluent : methanol/water (30/70) Comparion of Separative Selectivity (5) ー Hydrogen-bonding Interaction Graph chartー a (caffeine / benzene) Eluent : methanol/water (30/70)

Strong Retention and Unique Selectivity of Hydrosphere C18: Maltooligosaccharides Competitor LC Competitor WX 1. Glucose (G1) 2. Maltose (G2) 3. Maltotriose (G3) 4. Maltotetraose (G4) 5. Maltopentaose (G5) 6. Maltohexaose (G6) 7. Maltoheptaose (G7) Column : 150 X4.6 mmI.D. Eluent : water Flow rate : 1.0 mL/min Temperature : ambient (26℃) Detection : RI

Strong Retention and Unique Selectivity of Hydrosphere C18: Orotic acids Competitor SS Competitor LC Column : 150 X4.6 mmI.D. Eluent : 20mM H3PO4 Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm

Characteristics of Pro C18 RSTM High Carbon Content YMC-Pack Pro C18 RSTM is a polymeric-type phase bonded to high surface area silica, which contains the ODS groups at a high density. It shows a higher carbon content (22%) than the other conventional phases. Distinctive Separation Pro C18 RSTM recognizes subtle structural differences in hydrophobic compounds, e.g. structural isomers of terphenyl, to enable excellnet separations. The minimization of residual silanols achieved by the Lewis acid / base chemistry has resulted in less peak tailing of basic compounds. Improved pH-stability The polymeric bonding status and high carbon loading are considered to have resulted in improved pH-stability. It can be used even under highly demanding conditions.

Comparison of Stability under Strongly Acidic Conditions (pH 1, 70℃) % k' remaining (Butyl benzoate) * Recommended pH range: 1-12 Purged with water/acetonitrile/TFA (10/90/1) at 70℃ Pro C18 RSTM is highly stable under acidic conditions.

%N remaining (Methyl Benzoate) Comparison of Stability under Strongly Alkaline Conditions (pH 10, 30℃） %N remaining (Methyl Benzoate) Mobile phase: 20 mM H3BO4-NaOH(pH 9.8)/methanol (50/50) Temperature: 30℃ Life time of Pro C18 RSTM is threefold longer than that of monomeric ODS.